antibody

Glycogen Synthase Kinase 3 alpha (GSK3a)

G8170-41C

100 ul

polyclonal

domestic rabbit

nonconjugated

Glycogen Synthase Kinase 3 alpha (GSK3a)

rabbit

Pab

Antibodies

100 ul

IgG

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3a regulates the production of amyloid-b peptides, a major component of the plaques that accumulate with progression of Alzheimer’s disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-b production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by g secretase, blocking accumulation of amyloid-b peptides in the brains of mice that overproduce APP (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide (KLH-coupled) derived from the sequence of human GSK-3a.

Detects endogenous levels of total GSK-3a. Does not crossreact with recombinant GSK-3b. Species Crossreactivity: Human, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic peptide (KLH-coupled) derived from the sequence of human GSK-3a. Specificity: Detects endogenous levels of total GSK-3a. Does not crossreact with recombinant GSK-3b. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1) Welsh, G. I. et al. (1996) Trends Cell Biol. 6, 274–279. (2) Srivastava, A.K. and Pandey, S.K. (1998) Mol.Cell. Biochem. 182, 135–141. (3) Cross, D. A. et al. (1995) Nature 378, 785–789. (4) Nusse, R. (1997) Cell 89, 321–323. (5) Diehl, J. A. et al. (1998) Genes Dev. 12, 3499–3511. (6) Phiel, C. J. et al. (2003) Nature 423, 435–439.

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