antibody

Glycogen Synthase Kinase 3 alpha, beta (GSK3a/b, GSK3 beta, GSK-3 beta, GSK3b)

G8170-41A4

20 ul

monoclonal

domestic rabbit

nonconjugated

10E25(D75D3)

Glycogen Synthase Kinase 3 alpha, beta (GSK3a/b, GSK3 beta, GSK-3 beta, GSK3b)

rabbit

Mab

Antibodies

20 ul

10E25(D75D3)

IgG

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3 alpha and Ser9 of GSK-3 beta (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3 alpha regulates the production of amyloid-beta peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-beta production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by upsilon-secretase, blocking accumulation of amyloid-beta peptides in the brains of mice that overproduce APP (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu Mk Mo Rt

Synthetic peptide surrounding Gln269 of human GSK-3alpha.

Recognizes endogenous levels of total human GSK-3a and GSK-3b. Species Crossreactivity: mouse, rat, hamster and monkey

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E25(D75D3) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide surrounding Gln269 of human GSK-3alpha. Specificity: Recognizes endogenous levels of total human GSK-3a and GSK-3b. Species Crossreactivity: mouse, rat, hamster and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Welsh, G.I. et al. (1996) Trends Cell. Biol. 6, 274-279, 2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141, 3. Cross, D.A. et al. (1995) Nature 378, 785-789, 4. Nusse, R. (1997) Cell 89, 321-323, 5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511, 6. Phiel, C.J. et al. (2003) Nature 423, 435-9.

https://usbio-images.r.worldssl.net/prodimages/21/G8170-41A4_1.jpg