Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a) | US Biological G8170-40D5 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

catalog :

G8170-40D5

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

7H23(36E9)

reactivity :

human

product information

Catalog Number :

G8170-40D5

Product wo Prefix :

Glycogen Synthase Kinase 3 alpha, phosphorylated (Ser21) (GSK3a)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H23(36E9)

Isotype :

IgG

Desc1 :

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues around Ser21 of human GSK-3alpha.

Specificity :

Recognizes endogenous levels of human GSK-3alpha at ~51kD when phosphorylated at Ser21. Does not detect GSK-3beta when phosphorylated at Ser9. Species Crossreactivity: monkey, mouse and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H23(36E9) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues around Ser21 of human GSK-3alpha. Specificity: Recognizes endogenous levels of human GSK-3alpha at ~51kD when phosphorylated at Ser21. Does not detect GSK-3beta when phosphorylated at Ser9. Species Crossreactivity: monkey, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Welsh, G.I. et al. (1996) Trends Cell Biol. 6, 274–279. 2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135–141. 3. Cross, D.A. et al. (1995) Nature 378, 785–789. 4. Nusse, R. (1997) Cell 89, 321–323. 5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499–35.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/0/G8170-40D5_1.jpg

Picture4 Text :

IHC analysis of paraffin- embedded human breast carcinoma using G8170-40D5 in the presence of control peptide (left) or G8170-40R (right).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/0/G8170-40D5_5.jpg

Picture5 Text :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using G8170-40D5.