Glutamate | US Biological G3990-05A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Glutamate

catalog :

G3990-05A

quantity :

50 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H22

product information

Catalog Number :

G3990-05A

Product wo Prefix :

Glutamate

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

50 ul

Clone # USB :

3H22

Isotype :

IgG1

Desc1 :

A distinct step in inter-cellular communication involves termination of synaptic tranmission via the removal of neurotramsitters by specialized transporters. The regulated exocytotoxic release of neurotransmitters in reponse to neural activity requires storage within extracellular vesicles. In the nervous sytem, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secratory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks. Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2500. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

L-Glutamic acid (KLH).

Specificity :

Recognizes L- Glutamic acid when conjugated with glutaraldehye or immobilized on Sepharose. Crossreacts with Gly-Glu and Asp-Glu. Weak crossreactivity may be observed with D-Glu, L-Gln, L-Asn, b-Ala, Gly, 5-aminovaleric acid, g-aminobutyric acid (GABA) and Gly-Asp

Purity :

Ascites

Form :

Supplied as a liquid in PBS, 0.05% sodium azide.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3H22 Host: mouse Concentration: Not Determined Form: Supplied as a liquid in PBS, 0.05% sodium azide. Purity: Ascites Immunogen: L-Glutamic acid (KLH). Specificity: Recognizes L- Glutamic acid when conjugated with glutaraldehye or immobilized on Sepharose. Crossreacts with Gly-Glu and Asp-Glu. Weak crossreactivity may be observed with D-Glu, L-Gln, L-Asn, b-Ala, Gly, 5-aminovaleric acid, g-aminobutyric acid (GABA) and Gly-Asp Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Tanaka, K. et al., (1993) Neurosci. Lett. 16:149. 2. Shashidharan, P. et al., (1993) BBA 1216:161. 3. Rothstein, J. D. et al., Neron 13:713. 4. Rothstein, J.D., et al., (1995) Ann. Neruol. 38:73-84.