Glutamate | US Biological G3990-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Glutamate

catalog :

G3990-01

quantity :

2000Tests

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

product information

Catalog Number :

G3990-01

Product wo Prefix :

Glutamate

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

2000Tests

Isotype :

IgG

Desc1 :

Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Silver-intensified immunogold or fluorescence. Samples should be fixed with 0.1-2.5% glutaraldehyde for optimum detection. This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative). The minimum glutaraldehyde concentration is 0.00%. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Glutamate-glutaraldehyde

Specificity :

Recognizes Glutamate (L-Glutamate). Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable crossreactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/Lglutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Crossreactivity: D-glutamate, 1;20; free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue), 1:8.

Purity :

Purified

Form :

Supplied as a liquid in 100mM phosphate buffer, 1% sterile goat serum, 0.05% thimerosal

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Concentration: Not Determined Form: Supplied as a liquid in 100mM phosphate buffer, 1% sterile goat serum, 0.05% thimerosal Purity: Purified Immunogen: Glutamate-glutaraldehyde Specificity: Recognizes Glutamate (L-Glutamate). Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable crossreactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/Lglutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Crossreactivity: D-glutamate, 1;20; free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue), 1:8. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC

Storage Temperature :

4°C Do not freeze

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/G3990-01_1.jpg