antibody

Glucocorticoid Receptor, phosphorylated (Ser211)

G3030-07A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Glucocorticoid Receptor, phosphorylated (Ser211)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Glucocorticoid hormones control cellular proliferation and metabolism through their association with the glucocorticoid receptor (GR), a member of the intracellular receptor superfamily of transcriptional regulatory proteins (1). The GR zinc binding region (ZBR) harbors the DNA binding and protein dimerization functions of GR and is essential for regulation of all known GR target genes. In the absence of hormone, GR is localized to the cytoplasm in association with a molecular chaperone complex that interacts with the GR ligand binding domain (LBD). On hormone binding, GR releases the chaperone complex and translocates to the cell nucleus to associate with specific DNA sequences termed glucocorticoid response elements (GREs), and increases or represses transcription of specific target genes (2). It was demonstrated that GR-mediated transcriptional activation is modulated by phosphorylation (3–5). Although GR can be phosphorylated in the absence of hormone, it is further phosphorylated in conjunction with agonist (but not antagonist) binding. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, and receptor stability as well as receptor subcellular localization (3). Ser203 and Ser211 of human GR (corresponding to Ser224 and Ser232 of mouse GR) are shown to be phosphorylated to a greater extent in the presence of hormone. The cyclin-dependent kinases may be potential kinases that modify Ser203 and Ser211. Biochemical fractionation studies following hormone treatment indicated that the Ser203-phosphorylated form of the receptor was predominantly cytoplasmic, whereas Ser211-phosphorylated GR was found in the nucleus (3). Thus, Ser211 phosphorylation is a biomarker for activated GR in vivo. Applications: Suitable for use in Western Blot, Immunoprecipitation, ELISA, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunocytochemistry (IF): 1:1600 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphopeptide corresponding to residues surrounding serine 211 of human glucocorticoid receptor.

Recognizes endogenous levels of human glucocorticoid receptor only when phosphorylated at serine 211. Does not crossreact with other unrelated phosphorylated proteins.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding serine 211 of human glucocorticoid receptor. Specificity: Recognizes endogenous levels of human glucocorticoid receptor only when phosphorylated at serine 211. Does not crossreact with other unrelated phosphorylated proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IC IF IP WB

-20°C

1 Yamamoto, K.R. 1985 Annu. Rev. Genet 19, 209-252. 2 Necela, B.M. and Cidlowski, J.A. 2003 Trends Pharmacol. Sci. 24, 58-61. 3 Wang, Z. et al. 2002 J. Biol. Chem. 277, 26573-26580. 4 Rogatsky, I. et al. 1998 J. Biol. Chem. 273, 14315-14321. 5 Krstic, M.D. et al. 1997 Mol. Cell. Biol. 17, 3947-3954.

https://usbio-images.r.worldssl.net/prodimages/42/G3030-07A_1.jpg

Fig.2b: Confocal immunofluorescent analysis of HeLa cells, lambda phosphatase-treated using G3030-07A Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).