antibody

GLI3 (Transcriptional Activator GLI3, GLI3 Form of 190kD, GLI3-190, GLI3 Full Length Protein, GLI3FL)

G2035-54G

100 ug

polyclonal

domestic goat

nonconjugated

GLI3 (Transcriptional Activator GLI3, GLI3 Form of 190kD, GLI3-190, GLI3 Full Length Protein, GLI3FL)

goat

Pab

Antibodies

100 ug

IgG

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Ca Hu Mo Rt

Synthetic peptide corresponding to C-HNKRSKIKPDED, from GLI3, at the internal region of the protein (NP_000159.2).

Recognizes GLI3. Species sequence homology: Canine, human, mouse and rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-HNKRSKIKPDED, from GLI3, at the internal region of the protein (NP_000159.2). Specificity: Recognizes GLI3. Species sequence homology: Canine, human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E

-20°C

1. Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28.