This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Green Fluorescent Protein (GFP) (BSA & Azide Free)
catalog :
G2032-28DX
quantity :
100 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3F188
product information
Catalog Number :
G2032-28DX
Product wo Prefix :
Green Fluorescent Protein (GFP) (BSA & Azide Free)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
100 ul
Clone # USB :
3F188
Isotype :
IgG1,k
Desc1 :
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species-independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein-protein interactions in the yeast two-hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
MW of Antigen: 27kD
Epitope: Not determined
Applications:
Western Blotting (Not verified) The optimal dilution for a specific application should be determined by the investigator.
Positive Control: Purified recombinant GFP protein, transfected cell lines expressing GFP-tagged proteins.
Cellular Localization: Depends upon the localization of the parent protein tagged with GFP.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen :
Purified recombinant GFP protein.
Specificity :
Recognizes GFP (Green Fluorescent Protein). Species Crossreactivity: Proteins from all species which are tagged with GFP.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See G2032-28D
Concentration :
~1mg/ml
Desc2 :
Product Type: Mab
Isotype: IgG1,k
Clone No: 3F188
Host: mouse
Concentration: ~1mg/ml
Form: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See G2032-28D
Purity: Purified by Protein G affinity chromatography.
Immunogen: Purified recombinant GFP protein.
Specificity: Recognizes GFP (Green Fluorescent Protein). Species Crossreactivity: Proteins from all species which are tagged with GFP.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Storage Temperature :
-20°C
Reference :
1. Davis I, et al. (1995) Dev Biol, 170:726–729.
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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