antibody

Green Fluorescent Protein (GFP) discontinued

G2032-28D

500 ul

monoclonal

mouse

nonconjugated

3F188

Green Fluorescent Protein (GFP) discontinued

mouse

Mab

Antibodies

500 ul

3F188

IgG1,k

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species-independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein-protein interactions in the yeast two-hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments. MW of Antigen: 27kD Applications: Suitable for use in ELISA. Not tested for Western Blot. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Purified recombinant GFP protein, transfected cell lines expressing GFP-tagged proteins. Cellular Localization: Depends upon the localization of the parent protein tagged with GFP. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Purified recombinant GFP protein.

Recognizes GFP (Green Fluorescent Protein). Species Crossreactivity: Proteins from all species which are tagged with GFP.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G2032-28DX

~0.2mg/ml

Product Type: Mab Isotype: IgG1,k Clone No: 3F188 Host: mouse Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G2032-28DX Purity: Purified by Protein G affinity chromatography. Immunogen: Purified recombinant GFP protein. Specificity: Recognizes GFP (Green Fluorescent Protein). Species Crossreactivity: Proteins from all species which are tagged with GFP. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E

4°C (-20°C Glycerol)

1. Davis I, et al. (1995) Dev Biol, 170:726-729.