GADD45A (Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, DDIT1, GADD45) | US Biological G1017-19B product information

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product summary

company name :

US Biological

product type :

antibody

product name :

GADD45A (Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, DDIT1, GADD45)

catalog :

G1017-19B

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E22(D17E8)

reactivity :

human

product information

Catalog Number :

G1017-19B

Product wo Prefix :

GADD45A (Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, DDIT1, GADD45)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E22(D17E8)

Isotype :

IgG

Desc1 :

GADD45 alpha, beta and gamma are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha.

Specificity :

Recognizes endogenous levels of total human GADD45 alpha. Species crossreactivity: mouse, rat, monkey.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E22(D17E8) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha. Specificity: Recognizes endogenous levels of total human GADD45 alpha. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Wang, X.W. et al. (1999) Proc Natl Acad Sci USA 96, 3706-11, 2. Yang, Q. et al. (2000) J Biol Chem 275, 36892-8, 3. Zhao, H. et al. (2000) Exp Cell Res 258, 92-100, 4. Liebermann, D.A. and Hoffman, B. (2008) J Mol Signal 3, 15.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/47/G1017-19B_1.jpg