Gab1 (Grb2-Associated Binder1) | US Biological G1012 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Gab1 (Grb2-Associated Binder1)

catalog :

G1012

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

G1012

Product wo Prefix :

Gab1 (Grb2-Associated Binder1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

GRB-associated binder (Gab) is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs), such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane, undergoes phosphorylation and functions as a multiprotein assembling center (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of tyrosine 472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of tyrosines 307, 373 and 407 modulates the association to PLCgamma (8). Phosphorylation of tyrosines 627 and 659 are required for the binding and activation of protein tyrosine phosphatase SHP2 (6,9). Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic non-phosphopeptide corresponding to residues surrounding Tyr472 of human Gab1.

Specificity :

Detects endogenous levels of total human Gab1 protein at ~110kD. Does not crossreact with Gab2 or Gab3. Species Crossreactivity: mouse, rat and monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic non-phosphopeptide corresponding to residues surrounding Tyr472 of human Gab1. Specificity: Detects endogenous levels of total human Gab1 protein at ~110kD. Does not crossreact with Gab2 or Gab3. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Holgado-Madruga, M., et al., Nature 379: 560–564 (1996). 2. Weidner, K.M., et al., Nature 384: 173–176 (1996). 3. Takahashi-Tezuka, M., et al., Mol. Cell. Biol. 18: 4109–4117 (1998). 4. Ingham, R.J., et al., J. Biol. Chem. 276: 12,257–12,265 (2001). 5. Lehr, S., et al., Biochemistry 38: 151–159 (1999). 6. Rocchi, S., et al., Mol. Endocrinol. 12: 914–923 (1998). 7. Yu, C.F., et al., J. Biol. Chem. 276: 32,552–32,558 (2001). 8. Gual, P., et al., Oncogene 19: 1509–1518 (2000). 9. Cunnick, J.M., et al., J. Biol. Chem. 276: 24,380–24,387 (2001).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/G1012_1.jpg