antibody

Gab1, phosphorylated (Grb2-Associated Binder1) (Tyr627)

G1012-20

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Gab1, phosphorylated (Grb2-Associated Binder1) (Tyr627)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

GRB-associated binder (Gab) is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs), such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane, undergoes phosphorylation and functions as a multiprotein assembling center (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of tyrosine 472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of tyrosines 307, 373 and 407 modulates the association to PLCgamma (8). Phosphorylation of tyrosines 627 and 659 are required for the binding and activation of protein tyrosine phosphatase SHP2 (6,9). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1. Species sequence homology: mouse and hamster.

Recognizes endogenous levels of Tyr627 phosphorylated Gab1. This antibody may crossreact with phosphorylated Gab2 or Gab3 OR with activated receptor tyrosine kinases (RTKs). Species Crossreactivity: Human, monkey and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1. Species sequence homology: mouse and hamster. Specificity: Recognizes endogenous levels of Tyr627 phosphorylated Gab1. This antibody may crossreact with phosphorylated Gab2 or Gab3 OR with activated receptor tyrosine kinases (RTKs). Species Crossreactivity: Human, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Holgado-Madruga, M. et al. (1996) Nature 379, 560–564. (2) Weidner, K.M. et al. (1996) Nature 384, 173–176. (3) Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109–4117. (4) Ingham, R.J. et al. (2001) J. Biol. Chem. 276, 12257–12265. (5) Lehr, S. et al. (1999) Biochemistry 38, 151–159. (6) Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914–923. (7) Yu, C.F. et al. (2001) J. Biol. Chem. 276, 32552–32558. (8) Gual, P. et al. (2000) Oncogene 19, 1509–1518. (9) Cunnick, J.M. et al. (2001) J. Biol. Chem. 276, 24380–24387.

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