antibody

FOXC1 (Forkhead Box Protein C1, Forkhead-related Protein FKHL7, Forkhead-related Transcription Factor 3, FREAC-3, FKHL7, FREAC3)

F9045-49J

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse

FOXC1 (Forkhead Box Protein C1, Forkhead-related Protein FKHL7, Forkhead-related Transcription Factor 3, FREAC-3, FKHL7, FREAC3)

goat

Pab

Antibodies

100 ug

IgG

FOXC1, otherwise known as Forkhead box C1, a member of the Forkhead family of transcription factors, shown to be involved in the development of the eye. Studies have shown that FOXC1 plays an important role in the regulation of the FGF19-FGFR4-MAPK pathway involved in the maintenance of anterior segment structures within the eye. Studies have also shown that FOXC1 is essential for the aggressive phenotype of basal-like triple-negative breast cancer (TNBC), for which FOXC1 may prove to be a valuable diagnostic marker. Mutations in the FOXC1 gene are responsible for various ocular abnormalities including Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis anomaly (IGDA), and Peters anomaly. Applications: Suitable for use in ELISA, Western Blot, ChiP. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to RTSGAFVYDCSKF, from FOXC1, at C-terminal (NP_001444.2).

Recognizes FOXC1. Species Crossreactivity: Human and mouse.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to RTSGAFVYDCSKF, from FOXC1, at C-terminal (NP_001444.2). Specificity: Recognizes FOXC1. Species Crossreactivity: Human and mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

ChIP E WB

-20°C

1. Hayashi H, Kume T. Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells. PLoS One. 2008 Jun 11;3(6):e2401. 2. Berry FB, Skarie JM, Mirzayans F, Fortin Y, Hudson TJ, Raymond V, Link BA, Walter MA. FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Hum Mol Genet. 2008 Feb 15;17(4):490-505. 3. Tamimi Y, Skarie JM, Footz T, Berry FB, Link BA, Walter MA. FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. um Mol Genet. 2006 Nov 1;15(21):3229-40. 4. Berry FB, Lines MA, Oas JM, Footz T, Underhill DA, Gage PJ, Walter MA. Functional interactions between FOXC1 and PITX2 underlie the sensitivity to FOXC1 gene dose in Axenfeld-Rieger syndrome and anterior segment dysgenesis. Hum Mol Genet. 2006 Mar 15;15(6):905-19.

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