FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105, Transcription Factor 3A, TCF3A, TCF-3A) | US Biological F9045-02G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105, Transcription Factor 3A, TCF3A, TCF-3A)

catalog :

F9045-02G

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

product information

Catalog Number :

F9045-02G

Product wo Prefix :

FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105, Transcription Factor 3A, TCF3A, TCF-3A)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

FoxA1 is a 473aa, 49kD (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93-86aa identity with mouse and rat FoxA1, respectively. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.2-0.6ug/ml, observed in human lysates of cell line HepG2 on ~50kD bands Immunohistochemistry (Paraffin): 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mo Po Rt

Immunogen :

Synthetic peptide corresponding to C-GVYSRPVLNTS, from FOXA1, at C-terminal (NP_004487.2).

Specificity :

Recognizes human FOXA1. Species Crossreactivity: mouse. Species sequence homology: Bovine, porcine and rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-GVYSRPVLNTS, from FOXA1, at C-terminal (NP_004487.2). Specificity: Recognizes human FOXA1. Species Crossreactivity: mouse. Species sequence homology: Bovine, porcine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC IP WB

Storage Temperature :

-20°C

Reference :

1. McMullin RP, Dobi A, Mutton LN, Orosz A, Maheshwari S, Shashikant CS, Bieberich CJ. A FOXA1-binding enhancer regulates Hoxb13 expression in the prostate gland. Proc Natl Acad Sci USA 2010 Jan 5;107(1):98-103. 2. Taube JH, Allton K, Duncan SA, Shen L, Barton MC. Foxa1 functions as a pioneer transcription factor at transposable elements to activate Afp during differentiation of embryonic stem cells. J Biol Chem. 2010 May 21;285(21):16135-44. 3. Hua S, Kittler R, White KP. Genomic antagonism between retinoic acid and estrogen signaling in breast cancer. Cell. 2009 Jun 26;137(7):1259-71. 4. Eeckhoute J, Lupien M, Meyer CA, Verzi MP, Shivdasani RA, Liu XS, Brown M. Cell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancers. Genome Res. 2009 Mar;19(3):372-80. 5. Lupien M, Eeckhoute J, Meyer CA, Wang Q, Zhang Y, Li W, Carroll JS, Liu XS, Brown M. FoxA1 translates epigenetic signatures into enhancer-driven lineage-specific transcription. Cell. 2008 Mar 21;132(6):958-70. 6. Eeckhoute J, Carroll JS, Geistlinger TR, Torres-Arzayus MI, Brown M. A cell-type-specific transcriptional network required for estrogen regulation of cyclin D1 and cell cycle progression in breast cancer. Genes Dev. 2006 Sep 15;20(18):2513-26. 7. Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005 Jul 15;122(1):33-43. 8. Nguyen TT, Cho K, Stratton SA, Barton MC. Transcription factor interactions and chromatin modifications associated with p53-mediated, developmental repression of the alpha-fetoprotein gene. Mol Cell Biol. 2005 Mar;25(6):2147-57.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/46/F9045-02G_1.jpg