antibody

FRS2 alpha, Phosphorylated (Tyr436) (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

F7000-10

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

FRS2 alpha, Phosphorylated (Tyr436) (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine- phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). Activation of the FGFR leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2 alpha (SNT1) and FRS2 beta (SNT2) (2), which are phosphorylated by these RTKs. Once they are phosphorylated, they recruit SH2 domain-containing proteins including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic phosphopeptide corresponding to residues surrounding Tyr436 of human FRS2 alpha. SwissPort: Q8WU20. Species Sequence Homology (100%): rat

Recognizes endogenous levels of human FRS2 alpha at ~80-85kD only when phosphorylated at Tyr436. Does not crossreact with unrelated tyrosine-phosphorylated proteins. Species Crossreactivity: mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr436 of human FRS2 alpha. SwissPort: Q8WU20. Species Sequence Homology (100%): rat Specificity: Recognizes endogenous levels of human FRS2 alpha at ~80-85kD only when phosphorylated at Tyr436. Does not crossreact with unrelated tyrosine-phosphorylated proteins. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1) Kouhara, H. et al. (1997) Cell 89: 693–702. (2) Ong, S.H. et al. (2000) Mol. Cell. Biol. 20: 979–989. (3) Kontaridis, M.I. et al. (2002) Mol. Cell. Biol. 22: 3875–3891. (4) Xu, H. and Goldfarb, M. (2001) J. Biol. Chem. 276: 13049–13056. (5) Hadari, Y.R. et al. (1998) Mol. Cell. Biol. 18: 3966–3973.

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