FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT) | US Biological F7000-05C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

catalog :

F7000-05C

quantity :

100 ug

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

F7000-05C

Product wo Prefix :

FRS2 (Fibroblast Growth Factor Receptor Substrate 2, RS2, Suc-associated Neurotrophic Factor-induced Tyrosine-phosphorylated Target, SNT)

Host :

sheep

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

FRS2 (FGFR substrate 2; also known as SNT and FRS2-Alpha) is a 70-90kD member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1-6), a PTB domain that interacts with FGF and NGF receptors (aa 13-115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196-471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121-449, human FRS2 is 99% and 94% aa identical to canine and mouse FRS2, respectively. Applications: Suitable for use in Western Blot, Immunohistochemistry and Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human liver subjected to antigen retrieval. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to aa121-449 of human FRS2 expressed in E. coli.

Specificity :

Recognizes human FRS2.

Purity :

Purified by Immunoaffinity chromatography

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: sheep Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by Immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to aa121-449 of human FRS2 expressed in E. coli. Specificity: Recognizes human FRS2. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/F7000-05C_1.jpg