Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18) | US Biological F5750-15B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Folate receptor 1 (FOLR1) (folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

catalog :

F5750-15B

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

F5750-15B

Product wo Prefix :

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Folate receptor 1 (FOLR1; also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family. FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell. The human FOLR1 preproprecursor is 257aa in length. It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234). There are two forms of FOLR1. One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226). There is one potential alternate splice form that shows a five aa substitution for aa 121-205. Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 MCR-7 cells in a total volume ≤200ul. Wash cells in Flow Cytometry Staining Buffer (F4568-10) or other BSA-supplemented saline buffer. Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells.

Specificity :

Recognizes human FOLR1. Shows < 5% crossreactivity with recombinant human FOLR2 and FOLR3 in direct ELISAs.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells. Specificity: Recognizes human FOLR1. Shows < 5% crossreactivity with recombinant human FOLR2 and FOLR3 in direct ELISAs. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/F5750-15B_1.jpg