antibody

Flt3, phosphorylated (Tyr591) (Fms-like Tyrosine Kinase 3, CD135)

F4600-30

100 ul

monoclonal

mouse

nonconjugated

phosphorylated

3i15(54H1)

Flt3, phosphorylated (Tyr591) (Fms-like Tyrosine Kinase 3, CD135)

mouse

Mab

Phosphorylated

Antibodies

100 ul

3i15(54H1)

IgG2b

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), a member of the type III receptor tyrosine kinase family that includes c-Kit, PDGF and M-CSF receptors, is expressed on early hematopoietic progenitor cells, and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream cellular targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyrosine 591 is located in the juxtamembrane region of FLT3. It may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Detects a band at ~130kD for the nonglycosylated form of Flt3 and the glycosylated mature form is identified at ~160kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic phosphopeptide corresponding to the sequences surrounding Tyr591 of human Flt3.

Recognizes human FLT3 only when phosphorylated at tyrosine 591. May crossreact with some tyrosine-phosphorylated proteins. Species Crossreactivity: mouse

Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG2b Clone No: 3i15(54H1) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to the sequences surrounding Tyr591 of human Flt3. Specificity: Recognizes human FLT3 only when phosphorylated at tyrosine 591. May crossreact with some tyrosine-phosphorylated proteins. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9: 37-48. 2. Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30. 3. Namikawa, R. et al. (1996) Stem Cells 14: 388-395. 4. Beslu, N. et al. (1996) J. Biol. Chem. 271: 20075-20081. 5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem. Biophys. Res. Commun. 277: 195-199. 6. Zhang, S. et al. (1999) J. Leukoc. Biol. 65: 372-380. 7. Mizuki, M. et al. (2000) Blood 96: 3907-3914.

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