antibody

Fibroblast Growth Factor Receptor, phosphorylated, Tyr653/654 (FGFR)

F4305-03

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Fibroblast Growth Factor Receptor, phosphorylated, Tyr653/654 (FGFR)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain. Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues. Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosines 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling. The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to residues surrounding Tyr653/654 of human FGFR-1, the corresponding sequences are the same in FGFR-2, -3 and -4. MW: 120kD, 145kD. Species Sequence Homology: mouse and rat

Recognizes endogenous levels of human Tyr653/654 phosphorylated FGF receptors. Crossreacts with activated PDGF receptor and insulin/IGF-I receptors.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Tyr653/654 of human FGFR-1, the corresponding sequences are the same in FGFR-2, -3 and -4. MW: 120kD, 145kD. Species Sequence Homology: mouse and rat Specificity: Recognizes endogenous levels of human Tyr653/654 phosphorylated FGF receptors. Crossreacts with activated PDGF receptor and insulin/IGF-I receptors. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Powers, C.J. et al. (2000) Endocr. Relat. Cancer 7: 165–197. (2) Reilly, J.F. and Maher, P.A. (2001) J. Cell Biol. 275: 7771–7778. (3) Mohammadi, M. et al. (1996) Mol. Cell. Biol. 16: 977–989. (4) Mohammadi, M. et al. (1991) Mol. Cell. Biol. 11: 5068–5078. (5) Larsson, H. et al. (1999) J. Biol. Chem. 274: 25726–25734.

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