Fibroblasts | US Biological F4213 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Fibroblasts

catalog :

F4213

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2Q1507 (TE-7)

product information

Catalog Number :

F4213

Product wo Prefix :

Fibroblasts

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

2Q1507 (TE-7)

Isotype :

IgG1

Desc1 :

Applications: Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Other applications have not been tested. Recommended Dilutions: Immunofluorescence (Acetone fixed tissue): 1:100-1:200 FACS: 1:100 using fresh or acetone fixed tissue; do not use trypsin to prepare adherent cell lines, use a non-enzymatic detactment procedure. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Whole human thymic stoma cells.

Specificity :

Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.

Purity :

Purified by ion exchange chromatography.

Form :

Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 2Q1507 (TE-7) Host: mouse Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide Purity: Purified by ion exchange chromatography. Immunogen: Whole human thymic stoma cells. Specificity: Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC

Storage Temperature :

-20°C

Reference :

1.Haynes B.F. Adv. Immunol. 81–142(1984). 2. Strutman O. , Immunol. Rev.42: 138–184(1978). 3. Haynes B.F. et al J. Clin. Invest. 71: 9–14(1983),. 4. Haynes B.F. et al J. Exp. Med. 157: 907–920(1983). 5.Haynes B.F. et al J. Exp. Med. 159: 1149–1168 (1984),. 6. McFarland E.J. et al . J. Immunol. 133: 1241–1249 (1984). 7.Lobach D.F. et al , J. Immunol. 134: 250–257(1985). 8. Haynes B.F. , Clin. Res. 32: 500–507 (1984).