This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)
catalog :
F4149-77K
quantity :
100 ug
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
product information
Catalog Number :
F4149-77K
Product wo Prefix :
FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)
Host :
goat
Product Type :
Pab
Category :
Antibodies
Size1 :
100 ug
Isotype :
IgG
Desc1 :
FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:128,000
Western Blot: 0.01-0.1ug/ml, observed in human heart lysates is consistent with literature on ~30kD bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Ca Hu Mo
Immunogen :
Synthetic peptide corresponding to CTVSRVSHPVSKARK, from human FHL1, at the internal region of the protein (AAC72886.1).
Specificity :
Recognizes human FHL1. Species sequence homology: Canine and mouse.
Purity :
Purified by immunoaffinity chromatography
Form :
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Concentration :
~0.5mg/ml
Desc2 :
Product Type: Pab
Host: goat
Source: human
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity: Purified by immunoaffinity chromatography.
Immunogen: Synthetic peptide corresponding to CTVSRVSHPVSKARK, from human FHL1, at the internal region of the protein (AAC72886.1).
Specificity: Recognizes human FHL1. Species sequence homology: Canine and mouse.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E WB
Storage Temperature :
-20°C
Reference :
1. Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem. 1999 Sep 17;274(38):27083-91.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/34/F4149-77K_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
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