antibody

FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)

F4149-77J

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse, rat

FHL1 (Four and a Half LIM Domains Protein 1, FHL-1, Skeletal Muscle LIM-protein 1, SLIM, SLIM-1, SLIM1)

goat

Pab

Antibodies

100 ug

IgG

FHL1 is a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.05-0.3ug/ml, observed in human muscle lysates on ~30kD bands Immunohistochemistry (Paraffin): 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Hu Mo Po Rt

Synthetic peptide corresponding to CNKRFVFHNEQVY, from FHL1, at the internal region of the protein (NP_001440.1, NP_001153171.1).

Recognizes human FHL1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, porcine and rat.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to CNKRFVFHNEQVY, from FHL1, at the internal region of the protein (NP_001440.1, NP_001153171.1). Specificity: Recognizes human FHL1. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine, porcine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

1. Tang T, Lai NC, Hammond HK, Roth DM, Yang Y, Guo T, Gao MH. Adenylyl cyclase 6 deletion reduces left ventricular hypertrophy, dilation, dysfunction, and fibrosis in pressure-overloaded female mice. J Am Coll Cardiol. 2010 Apr 6;55(14):1476-86. 2. Schessl J, Taratuto AL, Sewry C, Battini R, Chin SS, Maiti B, Dubrovsky AL, Erro MG, Espada G, Robertella M, Saccoliti M, Olmos P, Bridges LR, Standring P, Hu Y, Zou Y, Swoboda KJ, Scavina M, Goebel HH, Mitchell CA, Flanigan KM, Muntoni F, Bönnemann CG. Clinical, histological and genetic characterization of reducing body myopathy caused by mutations in FHL1. Brain. 2009 Feb;132(Pt 2):452-64. 3. Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A. Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics. 2008 Mar;7(3):519-33.

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