FAK, phosphorylated (Tyr576/577) | US Biological F0019-58T product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

FAK, phosphorylated (Tyr576/577)

catalog :

F0019-58T

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

F0019-58T

Product wo Prefix :

FAK, phosphorylated (Tyr576/577)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases (2,3), PI3K and PLCgamma (4,5). The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the C-terminal region of FAK (6,7). Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Tyr576/577 of human FAK. Species sequence homology: chicken and xenopus.

Specificity :

Detects endogenous levels of Tyr576/577 phosphorylated FAK. Species Crossreactivity: human, mouse and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr576/577 of human FAK. Species sequence homology: chicken and xenopus. Specificity: Detects endogenous levels of Tyr576/577 phosphorylated FAK. Species Crossreactivity: human, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

(1) Parsons, J.T. et al. (2000) Oncogene 19: 5606–5613. (2) Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14: 1680–1688. (3) Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14: 147–155. (4) Chen, H.C. et al. (1996) J. Biol. Chem. 271: 26329–26334. (5) Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96: 9021–9026. (6) Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15: 954–963. (7) Schlaepfer, D.D. et al. (1994) Nature 372: 786–791.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/36/F0019-58T_1.jpg