FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) | US Biological F0019-57B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

catalog :

F0019-57B

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

F0019-57B

Product wo Prefix :

FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases (2,3), PI3K and PLCg (4,5). The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the carboxy-terminal region of FAK (6,7). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Bo Hu Mk Mo Po Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding amino acid residue 710 of human FAK. Species sequence homology: chicken

Specificity :

Detects endogenous levels of human FAK protein. Does not crossreact with other proteins. Species Crossreactivity: mouse, rat, monkey, bovine and porcine

Purity :

Purified by Protein A and peptide affinity chramotography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chramotography. Immunogen: Synthetic peptide corresponding to residues surrounding amino acid residue 710 of human FAK. Species sequence homology: chicken Specificity: Detects endogenous levels of human FAK protein. Does not crossreact with other proteins. Species Crossreactivity: mouse, rat, monkey, bovine and porcine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC IP WB

Storage Temperature :

-20°C

Reference :

(1) Parsons, J. T. et al. (2000) Oncogene 19: 5606–5613. (2) Schaller, M. D. et al. (1994) Mol. Cell. Biol. 14: 1680–1688. (3) Cobb, B. S. et al. (1994) Mol. Cell. Biol. 14: 147–155. (4) Chen, H. C. et al. (1996) J. Biol. Chem. 271: 26329–26334. (5) Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96: 9021–9026. (6) Calalb, M. B. et al. (1995) Mol. Cell. Biol. 15: 954–963. (7) Schlaepfer, D. D. et al. (1994) Nature 372: 786–791.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/F0019-57B_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using F0019-57B.