antibody

Ezh2 (Enhancer of Zeste Homolog 2, Polycomb group (PcG) Protein)

E9085B

100 ul

polyclonal

domestic rabbit

nonconjugated

Ezh2 (Enhancer of Zeste Homolog 2, Polycomb group (PcG) Protein)

rabbit

Pab

Antibodies

100 ul

IgG

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis. Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain. The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure. Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci. Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Po Rt

Synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein.

Recognizes endogenous levels of human Ezh2 protein at 98kD. The antibody may cross-react with Ezh2-related proteins. Species Crossreactivity: mouse, rat, porcine.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein. Specificity: Recognizes endogenous levels of human Ezh2 protein at 98kD. The antibody may cross-react with Ezh2-related proteins. Species Crossreactivity: mouse, rat, porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IP WB

-20°C

1. Seller, W.B. and Loda, M. (2002) Cancer Cell 2:349–350. 2. Visser, H.P. et al. (2001) Br. J. Haematol. 112:950–958. 3. Chen, H. et al. (1996) Genomics 38:30–37. 4. Tonini, T. et al. (2004) Oncogene 23:4930–4937. 5. Muller, J. et al. (2002) Cell 111:197–208. 6. Kleer, C.G. et al. (2003) Proc Natl. Acad. Sci. USA 100:11606–11611.

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