EZH1 (Histone-lysine N-methyltransferase EZH1, Enhancer Of Zeste Homolog 1, ENX-2, KIAA0388) | US Biological E9085-03A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EZH1 (Histone-lysine N-methyltransferase EZH1, Enhancer Of Zeste Homolog 1, ENX-2, KIAA0388)

catalog :

E9085-03A

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

product information

Catalog Number :

E9085-03A

Product wo Prefix :

EZH1 (Histone-lysine N-methyltransferase EZH1, Enhancer Of Zeste Homolog 1, ENX-2, KIAA0388)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

EZH1 encodes a protein of 747aa that displays 55%aa identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 2-6ug/ml, lysate of cell line MOLT4 observed on ~85kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mo

Immunogen :

Synthetic peptide corresponding to EIPNPPTSKCITY, from human EZH1, at N-terminal (NP_001982.2).

Specificity :

Recognizes human EZH1. Species sequence homology: Bovine and mouse.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to EIPNPPTSKCITY, from human EZH1, at N-terminal (NP_001982.2). Specificity: Recognizes human EZH1. Species sequence homology: Bovine and mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Abel KJ, Brody LC, Valdes JM, Erdos MR, McKinley DR, Castilla LH, Merajver SD, Couch FJ, Friedman LS, Ostermeyer EA, Lynch ED, King MC, Welcsh PL, Osborne-Lawrence S, Spillman M, Bowcock AM, Collins FS, Weber BL. Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1. Genomics. 1996 Oct 15;37(2):161-71.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/15/E9085-03A_1.jpg