Eukaryotic Translation Initiation Factor 4B (eIF4B) | US Biological E8949 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Eukaryotic Translation Initiation Factor 4B (eIF4B)

catalog :

E8949

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

E8949

Product wo Prefix :

Eukaryotic Translation Initiation Factor 4B (eIF4B)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Eukaryotic translation initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation; in plants, eIF4B is known to interact with the poly-(A) binding protein increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues at the N-terminus of human eIF4B.

Specificity :

Recognizes endogenous levels of human eIF4B independent of phosphorylation at ~80kD. Species Crossreactivity: mouse, rat and monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues at the N-terminus of human eIF4B. Specificity: Recognizes endogenous levels of human eIF4B independent of phosphorylation at ~80kD. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Le, H. et al. (1997) J. Biol. Chem. 272:16247–16255. 2. Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109:1467–1481. 3. Duncan, R.F. and Hershey, J.W. (1984) J. Biol. Chem. 259:11882–11889. 4. Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260:5493–5497. 5. Manzella, J.M. et al. (1991) J. Biol. Chem. 266:2383–2389. 6. Gingras, A.C. et al. (2001) Genes Dev. 15:807–826.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/45/E8949_1.jpg