eIF4G (Eukaryotic Translation Initiation Factor 4G) | US Biological E8949-56 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

eIF4G (Eukaryotic Translation Initiation Factor 4G)

catalog :

E8949-56

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C65H5

The same clone is also sold as:

Cell Signaling Technology: 2617

reactivity :

human

product information

Catalog Number :

E8949-56

Product wo Prefix :

eIF4G (Eukaryotic Translation Initiation Factor 4G)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

C65H5

Isotype :

IgG

Desc1 :

The initiation of translation is an important biological event, and a variety of factors contribute to this process. The eIF4 family of translation initiation factors bind to the 5' m7GTP mRNA cap and function to unwind the secondary structure of mRNA (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E and stimulates the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A (4), serving as an adaptor molecule in the eIF4 complex. Moreover, eIF4G also plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ala881 of human eIF4G.

Specificity :

Detects endogenous levels of total human eIF4G. Species Crossreactivity: mouse, monkey and rat

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: C65H5 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Ala881 of human eIF4G. Specificity: Detects endogenous levels of total human eIF4G. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Yan, R. and Rhoads, R.E. (1995) Genomics 26:394–398. 2. Morley, S.J. et al. (1997) RNA 3:1085–1104. 3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272:21677–21680. 4. De Gregorio, E. et al. (1998) RNA 4:828–836. 5. Ohlmann, T. et al. (1996) EMBO J. 15:1371–1382. 6. Borman, A.M. and Kean, K.M. (1997) Virology 237:129–136.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/18/E8949-56_1.jpg