Eukaryotic Translation Initiation Factor 4G (eIF4G) | US Biological E8949-54 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Eukaryotic Translation Initiation Factor 4G (eIF4G)

catalog :

E8949-54

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H21(C45A4)

reactivity :

human

product information

Catalog Number :

E8949-54

Product wo Prefix :

Eukaryotic Translation Initiation Factor 4G (eIF4G)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H21(C45A4)

Isotype :

IgG

Desc1 :

The initiation of translation is an important biological event, and a variety of factors contribute to this process. The eIF4 family of translation initiation factors bind to the 5' m7GTP mRNA cap and function to unwind the secondary structure of mRNA (1, 2). The amino-terminal portion of eIF4G physically associates with eIF4E and stimulates the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A (4), serving as an adaptor molecule in the eIF4 complex. Moreover, eIF4G also plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence around Gly188 of human eIF4G.

Specificity :

Recognizes endogenous levels of total human eIF4G protein. Species Crossreactivity: Monkey, mouse and rat.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H21(C45A4) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the sequence around Gly188 of human eIF4G. Specificity: Recognizes endogenous levels of total human eIF4G protein. Species Crossreactivity: Monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Yan, R. and Rhoads, R.E. (1995) Genomics 26:394-398. 2. Morley, S.J. et al. (1997) RNA 3:1085-1104. 3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272:21677-21680. 4. De Gregorio, E. et al. (1998) RNA 4:828-836. 5. Ohlmann, T. et al. (1996) EMBO J. 15:1371-1382. 6. Borman, A.M. and Kean, K.M. (1997) Virology 237:129-136.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/E8949-54_1.jpg

Picture4 Text :

Flow cytometric analysis of HeLa cells using E8949-54 (blue) compared to a nonspecific negative control antibody (red).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/E8949-54_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of HeLa cells using E8949-54 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).