Eukaryotic Translation Initiation Factor 4A (eIF4A) | US Biological E8949-39 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Eukaryotic Translation Initiation Factor 4A (eIF4A)

catalog :

E8949-39

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A12(C32B4)

reactivity :

human

product information

Catalog Number :

E8949-39

Product wo Prefix :

Eukaryotic Translation Initiation Factor 4A (eIF4A)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

8A12(C32B4)

Isotype :

IgG

Desc1 :

A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide derived from Met316 residues of human eIF4A protein (KLH).

Specificity :

Detects endogenous levels of total human eIF4A protein at ~48kD. Species Crossreactivity: mouse, rat and monkey.

Purity :

Serum

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A12(C32B4) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Serum Immunogen: Synthetic peptide derived from Met316 residues of human eIF4A protein (KLH). Specificity: Detects endogenous levels of total human eIF4A protein at ~48kD. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Rogers, G.W. et al. (2001) J. Biol. Chem. 276:12598–12608. 2. Rogers, G.W. et al. (1999) J. Biol. Chem. 274:12236–12244. 3. Svitkin, Y.V. et al. (2001) RNA 7:382–394. 4. Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8:1407–1414. 5. Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8:1319–1321.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/28/E8949-39_1.jpg