Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a) | US Biological E8949-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

catalog :

E8949-03

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

9B148(119A11)

reactivity :

human

product information

Catalog Number :

E8949-03

Product wo Prefix :

Eukaryotic Translation Initiation Factor 2, alpha, phosphorylated (Ser51) (eIF2a)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

9B148(119A11)

Isotype :

IgG

Desc1 :

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha induces potent phosphorylation of eIF2alpha at Ser51 (5,6) Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Dr Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha.

Specificity :

Recognizes endogenous human eIF2 alpha only when phosphorylated at Ser51. Does not recognize elF2alpha phosphorylated at other sites. Species crossreactivity: mouse, rat, monkey and D. melanogaster.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 9B148(119A11) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Specificity: Recognizes endogenous human eIF2 alpha only when phosphorylated at Ser51. Does not recognize elF2alpha phosphorylated at other sites. Species crossreactivity: mouse, rat, monkey and D. melanogaster. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC IP WB

Storage Temperature :

-20°C

Reference :

Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/E8949-03_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using E8949-03Rabbit mAb.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/E8949-03_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of HeLa cells, serum starved vs. Thapsigargin treated (30 minutes) using E8949-03 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).