Etk, phosphorylated (Tyr40) discontinued | US Biological E3580-10 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Etk, phosphorylated (Tyr40) discontinued

catalog :

E3580-10

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

E3580-10

Product wo Prefix :

Etk, phosphorylated (Tyr40) discontinued

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Etk, also known as BMX, is a member of the Bruton’s tyrosine kinase (Btk) family of tyrosine kinases (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of tyrosine 566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at tyrosine 40 (4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphorylated peptide (KLH coupled) corresponding to residues surrounding Tyr40 of human Etk. Molecular weight of Antigen: 76kD.

Specificity :

Recognizes endogenous levels of human Etk only when phosphorylated at tyrosine 40.

Purity :

Purified by Protein A and immunoaffinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic phosphorylated peptide (KLH coupled) corresponding to residues surrounding Tyr40 of human Etk. Molecular weight of Antigen: 76kD. Specificity: Recognizes endogenous levels of human Etk only when phosphorylated at tyrosine 40. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Tamagnone, L. et al. (1994) Oncogene 9:3683–3688. 2. Qiu, Y. and Kung, H.J. (2000) Oncogene 19:5651–5661. 3. Tsai, Y.T. et al. (2000) Mol. Cell. Biol. 20:2043–2054. 4. Chen, R. et al. (2001) Nat. Cell Biol. 3:439–444.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/41/E3580-10_1.jpg