Estrogen Receptor beta (ERb) | US Biological E3565-16P product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Estrogen Receptor beta (ERb)

catalog :

E3565-16P

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

E3565-16P

Product wo Prefix :

Estrogen Receptor beta (ERb)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry (frozen and paraffin sections): 2-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Rt

Immunogen :

Synthetic peptide corresponding to aa512-530 (CSPAEDSKSK EGSQNPQSQ) of C-terminal human ERb (estrogen receptor b).

Specificity :

Recognizes human ERb. Detects a band of ~55kD by Western blot. Does not cross-react with ERa. Species Crossreactivity: rat

Purity :

Purified by affinity chromatography.

Form :

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide. Purity: Purified by affinity chromatography. Immunogen: Synthetic peptide corresponding to aa512-530 (CSPAEDSKSK EGSQNPQSQ) of C-terminal human ERb (estrogen receptor b). Specificity: Recognizes human ERb. Detects a band of ~55kD by Western blot. Does not cross-react with ERa. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C

Reference :

1. F. Barchiesi, et al., (2004) J. Clin. Endocrinol. Metab. 89:2373.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/26/E3565-16P_1.jpg