antibody

Estrogen Receptor, alpha, phosphorylated (Ser104/106) (ER) discontinued

E3565-10B

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Estrogen Receptor, alpha, phosphorylated (Ser104/106) (ER) discontinued

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Estrogen receptor alpha (ER alpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen- dependent activation domains (AF-1 and AF-2, respectively), ER alpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER alpha activity (3,4). ER alpha is phosphorylated on multiple sites (5). Serines 104 and 106 are located in the N-terminal transcription activation function domain AF-1, and phosphorylation of Ser104/106 plays an important role in regulating ER alpha activity (5). Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Eq Hu Mo

Synthetic phospho-Ser104/106 peptide (KLH coupled) corresponding to residues surrounding Ser104/106 of human ER alpha.

Recognizes overexpressed levels of ER alpha only when phosphorylated at Ser104/106. Does not crossreact with phosphorylated ER isoform beta. Species Crossreactivity: Predicted to react with mouse and equine phosphorylated ER alpha based on sequence homology.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phospho-Ser104/106 peptide (KLH coupled) corresponding to residues surrounding Ser104/106 of human ER alpha. Specificity: Detects overexpressed levels of Ser104/106 phosphorylated ER alpha. Detects ER alpha only when phosphorylated at Ser104/106. It does not crossreact with phosphorylated ER isoform beta. Species Crossreactivity: Predicted to react with the specified phosphorylated motif from mouse and equine based on sequence homology. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IC IF WB

4°C (-20°C Glycerol)

1. Mangelsdorf, D.J. et al. (1995) Cell 83:835–839. 2. Glass, C.K. and Rosenfeld, M.G. (2000) Gene Dev. 14:121–141. 3. Chen, D. et al. (1999) Mol. Cell. Biol. 19:1002–1015. 4. Campbell, R.A. et al. (2001) J. Biol. Chem. 276:9817–9824. 5. Rogatsky, I. et al. (1999) J. Biol. Chem. 274:22296–22302.

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