antibody

ERK5, phosphorylated (Thr218, Tyr220) (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)

E3452-59A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

ERK5, phosphorylated (Thr218, Tyr220) (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Erk5, also known as BMK1 (big mitogen-activated kinase 1), has a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the N-terminal kinase domain with Erk1/2. However, the long C-terminal domain makes Erk5 a unique MAP kinase. Erk5 is activated by a wide variety of extracellular signals, including tyrosine receptor kinases, G-protein-coupled receptors as well as osmotic and oxidative stress (1,2). The direct upstream kinase of Erk5 is defined as MEK5 (1). MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3-5). Growing evidence indicates that the Erk5 pathway is involved in proliferation/differentiation regulation, oncogenesis and neuronal development (1,6-8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000; Mr ~115kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

EC=2.7.11.24

Synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5

Detects immunoprecipitated or transfected levels of human Erk5 phosphorylated at threonine 218/tyrosine 220. Crossreacts with phosphorylated Erk1 and Erk2. It does not crossreact with phosphorylated p38 MAPK or SAPK/JNK. Species Crossreactivity: mouse, monkey and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See E3452-59A1.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See E3452-59A1. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5 Specificity: Detects immunoprecipitated or transfected levels of human Erk5 phosphorylated at threonine 218/tyrosine 220. Crossreacts with phosphorylated Erk1 and Erk2. It does not crossreact with phosphorylated p38 MAPK or SAPK/JNK. Species Crossreactivity: mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Kato, Y., et al., (1997) EMBO J. 16:7054–7066. 2. Fukuhara, S., et al., (2000) J. Biol. Chem. 275:21,730–21,736. 3. Chao, T., et al., (1999) J. Biol. Chem. 274:36,035–36,038. 4. Sun, W., et al., (2001) J. Biol. Chem. 276:5093–5100. 5. Chiariello, M., et al., (2000) Mol. Cell. Biol. 20:1747–1758. 6. Dinev, D., et al., (2001) EMBO Rep. 2:829–834. 7. Esparis-Ogando, A., et al., (2002) Mol. Cell. Biol. 22:270–285. 8. Watson, F.L., et al., (2001) Nat. Neurosci. 4:981–988.

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