ErbB4 (c-erbB4, HER4) discontinued | US Biological E3451-40 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

ErbB4 (c-erbB4, HER4) discontinued

catalog :

E3451-40

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

0.T.196

reactivity :

human

product information

Catalog Number :

E3451-40

Product wo Prefix :

ErbB4 (c-erbB4, HER4) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

0.T.196

Isotype :

IgG2a

Desc1 :

ErbB4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. ErbB4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. ErbB4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor. Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The ErbB4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif, known as the PDZ domain. Applications: Suitable for use in Immunoprecipitation, Biological Blockade of Heregulin Activity, Neutralization and Flow Cytometry. Not recommended for Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 10ug immunoprecipitates erbB-4/HER-4 from 500ug of RIPA lysate prepared from hEGF stimulated A431 cells, as demostrated by subsequent immunoblot of the immunoprecipitate with P4078-05. The immune complex was subsequently resolved by electrophoresis and transferred to nitrocellulose and probed with P4078-05 at 1ug/ml. Proteins were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system. Biological Blockade of Heregulin Activity: Reported to inhibit heregulin activity as measured by mitogenicity and radio-ligand binding assays. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Extracellular domain of recombinant human erbB-4/HER-4.

Specificity :

Recognizes human c-erbB-4/HER-4 at ~185kD, but not EGF receptor, erbB-2/HER-2 or c-erbB-3/ HER-3.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a liquid in 10mM PBS, pH 7.4.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 0.T.196 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in 10mM PBS, pH 7.4. Purity: Purified by Protein A affinity chromatography. Immunogen: Extracellular domain of recombinant human erbB-4/HER-4. Specificity: Recognizes human c-erbB-4/HER-4 at ~185kD, but not EGF receptor, erbB-2/HER-2 or c-erbB-3/ HER-3. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IP N

Storage Temperature :

-20°C

Reference :

11. Chen, X., et al., J. Biol. Chem. 271: 7620-7629 (1996).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/2/E3451-40_1.jpg