antibody

ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)

E3451-26B

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The ErbB2 (HER2) proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1). ErbB2 does not have any known ligand; however, the kinase activity of ErbB2 can be activated without any ligand if it is overexpressed and by heteroassociation with other members of the ErbB family (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for breast cancer and other carcinomas. The therapeutic potential may result from directing ErbB2 to a c-Cblregulated proteolytic pathway. Tyrosine 877 of ErbB2 is homologous to pp60c-Src Tyr416 located in the kinase domain. Phosphorylation of this site may be involved in regulation of ErbB2 biological activity. Tyrosine 1248 of ErbB2 is one of the major autophosphorylation sites in this molecule. The phosphorylation of this site couples ErbB2 to the Ras- Raf-MAP kinase signal transduction pathway (5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Tyr877 of human ErbB2.

Recognizes endogenous levels of human ErbB2 only when phosphorylated at tyrosine 877 at ~185kD. May crossreact with other ErbB family members (e.g., EGF receptor) when their homologous tyrosines are phosphorylated, but it does not crossreact with other tyrosine-phosphorylated proteins. Species sequence homology: mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr877 of human ErbB2. Specificity: Recognizes endogenous levels of human ErbB2 only when phosphorylated at tyrosine 877 at ~185kD. May crossreact with other ErbB family members (e.g., EGF receptor) when their homologous tyrosines are phosphorylated, but it does not crossreact with other tyrosine-phosphorylated proteins. Species sequence homology: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Muthuswamy, S.K. et al. (1999) Mol. Cell. Biol. 19:6845–6857. 2. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1500–1504. 3. Dittadi, R. and Gion, M. (2000) J. Natl. Cancer Inst. 92:1443–1444. 4. Klapper, L.N. et al. (2000) Cancer Res. 60:3384–3388. 5. Kwon, Y.K. et al. (1997) J. Neurosci. 17:8293–8299.

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