Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992) | US Biological E3375-53C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992)

catalog :

E3375-53C

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

E3375-53C

Product wo Prefix :

Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLCgamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr992 of human EGF receptor.

Specificity :

Recognizes human overexpressed EGF receptor only when phosphorylated at Tyr992 at 175kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species sequence homology: Mouse and rat-100%.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr992 of human EGF receptor. Specificity: Recognizes human overexpressed EGF receptor only when phosphorylated at Tyr992 at 175kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species sequence homology: Mouse and rat-100%. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11, 184-189. 2. Zwick , E. et al. (1999) Trends Pharmacol. Sci. 20, 408-412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-1054. 4. Hubbard, S.R. et al. (1994) Nature 372, 746-754. 5. Biscardi, J.S. et al. (1999) J. Biol. Chem. 274, 8335-8343. 6. Emlet, D.R. et al. (1997) J. Biol. Chem. 272, 4079-4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4, 1029-1040. 8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-1866. 9. Rojas, M. et al. (1996) J. Biol. Chem. 271, 27456-27461. 10. Iordanov, M. S. et al. (2002) Mol. Cell. Biol. 22, 5380-5394.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/11/E3375-53C_1.jpg

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/11/E3375-53C_6.jpg

Picture6 Text :

Immunofluorescent staining of untreated (left) and EGF treated (right) A431 cells, using E3375-53C.