antibody

Epidermal Growth Factor Receptor, phosphorylated (Tyr1045) (erbB, EGFR)

E3375-53

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Epidermal Growth Factor Receptor, phosphorylated (Tyr1045) (erbB, EGFR)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry . Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (IF-IC): 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Rt

Synthetic phosphopeptide corresponding to residues surrounding Tyr1045 of human EGF receptor.

Recognizes human EGF receptor only when phosphorylated at Tyr1045. May crossreact with other activated EGF receptor family members such as ErB2. Species crossreactivity: rat.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined. Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1045 of human EGF receptor. Specificity: Recognizes human EGF receptor only when phosphorylated at Tyr1045. May crossreact with other activated EGF receptor family members such as ErB2. Species crossreactivity: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IC IF IHC WB

-20°C

1. Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11: 184–189. 2. Zwick , E. et al. (1999) Trends Pharmacol. Sci. 20, 408–412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73: 1051–1054. 4. Hubbard, S.R. et al. (1994) Nature 372: 746–754. 5. Biscardi, J.S. et al. (1999) J. Biol. Chem. 274: 8335–8343. 6. Emlet, D.R. et al. (1997) J. Biol. Chem. 272: 4079–4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4: 1029–1040. 8. Ettenberg, S.A. et al. (1999) Oncogene 18:1855–1866. 9. Rojas, M. et al. (1996) J. Biol. Chem. 271: 27456–27461. 10. Iordanov, M.S. et al. (2002) Mol. Cell. Biol. 22: 5380–5394.

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