antibody

Epidermal Growth Factor Receptor (erbB, EGFR)

E3375-01J

100 ul

monoclonal

domestic rabbit

nonconjugated

9j122(C74B9)

Epidermal Growth Factor Receptor (erbB, EGFR)

rabbit

Mab

Antibodies

100 ul

9j122(C74B9)

IgG

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCgamma-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Recombinant fusion protein containing the cytoplamic domain of human EGF receptor.

Detects endogenous levels of total human EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family.

Serum

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 9j122(C74B9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Serum Immunogen: Recombinant fusion protein containing the cytoplamic domain of human EGF receptor. Specificity: Detects endogenous levels of total human EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11:184-189. 2. Zwick, E. et al. (1999) Trends Pharmacol. Sci. 20:408- 412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73:1051-1054. 4. Hubbard, S.R. et al. (1994) Nature 372:746-754. 5. Biscardi, J.S. et al. (1999) J. Biol. Chem. 274:8335–8343. 6. Emlet, D.R. et al. (1997) J. Biol. Chem. 272:4079–4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4:1029–1040. 8. Ettenberg, S.A. et al. (1999) Oncogene 18:1855–1866. 9. Rojas, M. et al. (1996) J. Biol. Chem. 271:27456–27461. 10. Feinmesser, R.L. et al. (1999) J. Biol. Chem. 274:16168–16173.

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