Epidermal Growth Factor Receptor (erbB, EGFR) (APC) | US Biological E3375-01B1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Epidermal Growth Factor Receptor (erbB, EGFR) (APC)

catalog :

E3375-01B1

quantity :

100Tests

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

8H184

reactivity :

human

product information

Catalog Number :

E3375-01B1

Product wo Prefix :

Epidermal Growth Factor Receptor (erbB, EGFR) (APC)

Host :

rat

Product Type :

Mab

Conjugate :

APC

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

8H184

Isotype :

IgG2a

Desc1 :

The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins that have an extracellular domain containing two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain that has a membrane-proximal tyrosine kinase domain, and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF, and neuregulin-2 alpha, in the absence of a co-receptor. Ligand binding induces EGF R homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation, and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis.1-3 Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

EC=2.7.10.1, ERBB1, MENA

Immunogen :

Recombinant protein corresponding to Leu25-Ser645 from human EGF R/ErbB1, expressed in Mouse myeloma cell line NS0.

Specificity :

Recognizes human EGFR.

Purity :

Purified by Protein G affinity chromatography from cell culture supernatant.

Form :

Supplied as a liquid in saline, BSA, sodium azide. Labeled with APC.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 8H184 Host: rat Source: human Concentration: Not determined Form: Supplied as a liquid in saline, BSA, sodium azide. Labeled with APC. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to Leu25-Ser645 from human EGF R/ErbB1, expressed in Mouse myeloma cell line NS0. Specificity: Recognizes human EGFR. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do not freeze

Reference :

1. Daly, R.J., (1999) Growth Factors, 16:255-263. 2. Schlessinger, J., (2000) Cell. 103:211-225. 3. Maihle, N.J. et al., (2002) Cancer Treat. Res. 107:247-258.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/41/E3375-01B1_1.jpg