antibody

Ephrin B, phosphorylated (Tyr324/329)

E3371-01A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Ephrin B, phosphorylated (Tyr324/329)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, and EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases and cancers (3). Both ephrin A and ephrin B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as “reverse signaling” whereby their cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as a major tyrosine phosphorylation sites of ephrin B1 in vivo (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphotyrosine peptide corresponding to residues surrounding Tyr324/329 of human ephrin B1.

Recognizes transfected levels of human Ephrin B at ~59kD only when phosphorylated at Tyr324/329. Cossreacts with ephrins B1, B2 and B3. Does not crossreact with other tyrosine-phosphorylated proteins.

Purified by protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by protein A and peptide affinity chromatography. Immunogen: Synthetic phosphotyrosine peptide corresponding to residues surrounding Tyr324/329 of human ephrin B1. Specificity: Recognizes transfected levels of human Ephrin B at ~59kD only when phosphorylated at Tyr324/329. Cossreacts with ephrins B1, B2 and B3. Does not crossreact with other tyrosine-phosphorylated proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Wilkinson, D.G. (2000) Int. Rev. Cytol. 196:177–244. 2. Klein, R. (2001) Curr. Opin. Cell Biol. 13:196–203. 3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19:5614–5619. 4. Holder, N. and Klein, R. (1999) Development 126:2033–2044. 5. Bruckner, K. et al. (1997) Science 275:1640–1643. 6. Palmer, A. et al. (2002) Mol. Cell 9:1–20. 7. Kalo, M. S. et al. (2001) J. Biol. Chem. 276:38940–38948.

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