antibody

ENO1 (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1)

E3222-75J

100 ul

monoclonal

mouse

nonconjugated

13A3

ENO1 (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1)

mouse

Mab

Antibodies

100 ul

13A3

IgG2b,k

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:10,000 Immunoprecipitation: 2ul Optimal dilutions to be determined by the researcher. Positive Control: A431 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Recombinant corresponding to human NNE expressed in E.coli.

Recognizes human NNE.

Ascites

Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG2b, k Clone No: 13A3 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in HEPES, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Recombinant corresponding to human NNE expressed in E.coli. Specificity: Recognizes human NNE. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Ray R., Miller D.M. (1991) Mol. Cell. Biol. 11:2154-2161. 2. Sugahara T. Et al. (1992) Cytotechnology 10:137-146. 3. Ghosh A.K. Et al. (1999) Mol. Cell. Biol. 19:2880-2886. 4. Feo S. Et al. (2000) FEBS Lett. 473:47-52. 5. Lopez-Alemany R. Et al (2003) Am. J. Hematol. 72:234-242. 6. Arza B. Et al. (1997) Thromb. Haemost. 78:1097-1103.

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