antibody

Endothelin B Receptor (ET-B; Nonselective Type Endothelin Receptor, Ednrb, ETB, ETRB)

E3110-02H

100 ug

polyclonal

domestic sheep

nonconjugated

Endothelin B Receptor (ET-B; Nonselective Type Endothelin Receptor, Ednrb, ETB, ETRB)

sheep

Pab

Antibodies

100 ug

IgG

EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2 and 3, and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75aa N-terminal extracellular region (aa27-101), and a 44aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa27-101. One shows a 90aa N-terminal extension, a second shows the same 90aa N-terminal substitution coupled with a deletion of aa268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa27-101, human EDNRB shares 67% and 97% aa identity with mouse and canine EDNRB, respectively. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells using A172 human glioblastoma cell line. Western Blot: 1ug/ml using HEK293 human embryonic kidney cell line, A172 human glioblastoma cell line and Raji human Burkitt’s lymphoma cell line. Immunocytochemistry: 5-15ug/ml using Immersion fixed A172 human glioblastoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to Gly27-Lys101 from human Endothelin B Receptor, expressed in E. coli.

Recognizes human Endothelin B Receptor.

Purified by immunoaffinity chromatography

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: sheep Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Gly27-Lys101 from human Endothelin B Receptor, expressed in E. coli. Specificity: Recognizes human Endothelin B Receptor. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IC WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/8/E3110-02H_1.jpg