Endoplasmic Reticulum Protein 72 (ERp72, PDIA4, Protein Disulfide Isomerase A4) | US Biological E2291-77C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Endoplasmic Reticulum Protein 72 (ERp72, PDIA4, Protein Disulfide Isomerase A4)

catalog :

E2291-77C

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

E2291-77C

Product wo Prefix :

Endoplasmic Reticulum Protein 72 (ERp72, PDIA4, Protein Disulfide Isomerase A4)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 72 (ERp72), constitute the PDI family (3,4). ERp72 contains three thioredoxin homology domains (3) and plays a role in the formation and isomerization of disulfide bonds on secretory proteins (3,4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the sequence of human ERp72.

Specificity :

Recognizes endogenous levels of total human ERp72. Species Crossreactivity: mouse, monkey and rat

Purity :

Purified by peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the sequence of human ERp72. Specificity: Recognizes endogenous levels of total human ERp72. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92:145–8. 2. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6:28–32. 3. Mazzarella, R.A. et al. (1990) J Biol Chem 265:1094–101. 4. Satoh, M. et al. (2005) Cell Stress Chaperones 10:278–84.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/40/E2291-77C_1.jpg