antibody

Emerin (Ala212) discontinued

E2257-01

100 ul

polyclonal

domestic rabbit

nonconjugated

Emerin (Ala212) discontinued

rabbit

Pab

Antibodies

100 ul

IgG

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and Aand B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to beta-catenin and inhibits its nuclear accumulation (8). Emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1alpha , and a transcription repressor. Recent studies demonstrated that Emerin is required for HIV-1 infectivity (9). It is speculated that patients suffering from Emery-Dreifuss muscular dystrophy may have immunity to or show an irregular infection pattern to HIV-1. Mutations in Emerin are a major cause of X-linked recessive Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding Ala212 of human Emerin.

Detects endogenous levels of total human Emerin protein.

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ala212 of human Emerin. Specificity: Detects endogenous levels of total human Emerin protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Nagano, A. et al. (1996) Nat. Genet. 12:254–259. 2. Manilal, S. et al. (1998) Biochem. Biophys. Res. Com- mun. 249:643–647. 3. Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267:709–714. 4. Lee, K.K. et al. (2001) J. Cell Sci. 114:4567–4573. 5. Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17:1154–1163. 6. Holaska, J.M. et al. (2003) J. Biol. Chem. 278:6969–6975. 7. Haraguchi, T. et al. (2004) Eur. J. Biochem. 271:1035–1045. 8. Markiewicz, E. et al. (2006) EMBO J. 25:3275–3285. 9. Jacque, J.M. and Stevenson, M. (2006) Nature 441:641–645. 10. Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288:676–680.

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