antibody

Elk-1

E2240

100 ul

polyclonal

domestic rabbit

nonconjugated

Elk-1

rabbit

Pab

Antibodies

100 ul

IgG

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1XTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide (KLH coupled) derived from the sequence of human Elk-1.

Detects endogenous (phosphorylation-state independent) levels of total Elk-1 protein. Species Crossreactivity: Human, mouse and rat.

Purified by Protein A affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservatives added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservatives added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) derived from the sequence of human Elk-1. Specificity: Detects endogenous (phosphorylation-state independent) levels of total Elk-1 protein. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Marais, R. et al. (1993) Cell 73, 381-393. 2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14, 4815-4824. 3. Hill, C.S. and Treisman, R. (1995) Cell 80, 199-211. 4. Cavigelli, M. et al. (1995) EMBO J. 14, 5957-5964. 5. Whitmarsh, A.J. et al. (1995) Science 269, 403-407. 6. Aplin, A.E. et al. (2001) J. Cell Biol. 153, 273-282. 7. Berman, D.E. et al. (1998) J. Neurosci. 18, 10037-10044. 8. Majka, M. et al. (2000) Blood 96, 4142-4151. 9. Stewart, S. and Guan, K.L. (2000) J. Biol. Chem. 275, 8854-8862. 10. Wang, D. and Richmond, A. (2001) J. Biol. Chem. 276, 3650-3659.

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Immunohistochemical analyssis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Elk-1 Antibody.