antibody

Elk-1, phosphorylated (Ser383)

E2240-02

100 ul

monoclonal

mouse

nonconjugated

phosphorylated

3A29(2B1)

Elk-1, phosphorylated (Ser383)

mouse

Mab

Phosphorylated

Antibodies

100 ul

3A29(2B1)

IgG1

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1–3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000 incubate membrane with diluted antibody in 5% non-fat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser383 of human Elk-1. Species sequence homology: Mouse and rat.

Detects overexpressed levels of human Elk-1 protein only when phosphorylated at Ser383. Detects ≤ 5ng of EIk-1 phosphorylated at Ser383 and will not react with ≤ 1ug of non-phosphorylated EIk-1 protein.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 3A29(2B1) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser383 of human Elk-1. Species sequence homology: Mouse and rat. Specificity: Detects overexpressed levels of human Elk-1 protein only when phosphorylated at Ser383. Detects ≤ 5ng of EIk-1 phosphorylated at Ser383 and will not react with ≤ 1ug of non-phosphorylated EIk-1 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Marais, R. et al. (1993) Cell 73:381–393. 2. Kortenjann, M. et al. (1994) Mol. Cell. Biol. 14:4815–4824. 3. Hill, C.S. and Treisman, R. (1995) Cell 80:199–211. 4. Cavigelli, M. et al. (1995) EMBO J. 14:5957–5964. 5. Whitmarsh, A.J. et al. (1995) Science 269:403–407.

https://usbio-images.r.worldssl.net/prodimages/23/E2240-02_1.jpg

https://usbio-images.r.worldssl.net/prodimages/23/E2240-02_6.jpg

Confocal images of immunohistochemical staining of rat striatum regions, control (left) or 15 minute transient cerebral ischemia followed by 30 minutes of reperfusion (right), usingE2240-02.