antibody

eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, Argonaute 1, AGO1, Golgi Endoplasmic Reticulum Protein 95kD, GERP95, Q99, Putative RNA-binding Protein Q99)

E2237-25B

100 ul

polyclonal

domestic rabbit

nonconjugated

eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, Argonaute 1, AGO1, Golgi Endoplasmic Reticulum Protein 95kD, GERP95, Q99, Putative RNA-binding Protein Q99)

rabbit

Pab

Antibodies

100 ul

IgG

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4) and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Lys400 of human eIF2C1.

Recognizes endogenous levels of total human eIF2C1 at ~97kD. Species Crossreactivity: Mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Lys400 of human eIF2C1. Specificity: Recognizes endogenous levels of total human eIF2C1 at ~97kD. Species Crossreactivity: Mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Peters, L. and Meister, G. (2007) Mol Cell 26, 611-23. 2. Filipowicz, W. et al. (2008) Nat Rev Genet 9, 102-14. 3. Caplen, N.J. et al. (2001) Proc Natl Acad Sci USA 98, 9742-7. 4. Mourelatos, Z. et al. (2002) Genes Dev 16, 720-8. 5. Girard, A. et al. (2006) Nature 442, 199-202.

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