antibody

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

E2215-30C

100 ul

monoclonal

domestic rabbit

nonconjugated

9j17(44D5)

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

rabbit

Mab

Antibodies

100 ul

9j17(44D5)

IgG

EGR family members are transcriptional factors with 3 repetitive Zinc finger DNA binding domains which bind to EGR response element (ER) and regulate target gene expression. The expression of EGR family members is induced by growth factors. Among the family members, EGR1 expression has been specifically shown to be induced by NGF. EGR1 expression further activates transcription of other signaling molecules including CDK5 and tyrosine hydroxylase to exert long term effects on neural cell growth and differentiation. Zif268 is a mammalian transcription factor that is now officially known as Egr1 (Early Growth Response Protein 1). It was also named Krox-24, NGFI-A, TIS8, and ZENK. It was originally discovered in mouse. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a tumor suppressor gene. The DNA binding domain of Zif268 consists of three zinc finger domains of the Cys2His2 type. The crystal structure of DNA bound by the zinc finger domain of Zif268 was solved in 1991, which greatly aided early research in zinc finger DNA-binding domains. The human Zif268/EGR1 protein contains (in its unprocessed form) 543 amino acids with a molecular weight of 57.5kD, and the gene is located on the chromosome 5. Zif268 binds the DNA sequence 5'-GCG[G/T]GGGCG-3'. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity. Zif268 has also been found to regulate the expression of synaptobrevin II (a protein important for synaptic exocytosis). Zif268 has been shown to interact with NAB1, CEBPB, EP300, CREB binding protein, PSMA3 and P53. Applications: Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 (Mr 75kD) Immunofluorescence (IF-IC): 1:400 Immunoprecipitation: 1:50 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide derived from the N-terminal sequence of human EGR1.

Detects endogenous levels of total EGR1. Species Crossreactivity: human, mouse and rat. Expected to cross-react with bovine due to sequence homolgy.

Purified by immunoaffinity chromatography

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 9j17(44D5) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide derived from the N-terminal sequence of human EGR1. Specificity: Detects endogenous levels of total EGR1. Species Crossreactivity: human, mouse and rat. Expected to cross-react with bovine due to sequence homolgy. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IP WB

-20°C

1. O’Donovan, K.J., et al., (1999) Trends Neurosci. 22:167–173. 2. Harada, T., et al., (2001) Nat. Cell Biol. 3:453–459. 3. Papanikolaou, N.A. & Sabban, E.L., (2000) J. Biol. Chem. 275: 26,683–26,689.

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